/manager/Index ${session.getAttribute("locale")} 5 A comparison of in vivo viral targeting systems identifies adeno-associated virus serotype 9 (AAV9) as an effective vector for genetic manipulation of Leydig cells in adult mice /manager/Repository/uon:38049 Wed 28 Jul 2021 16:00:45 AEST ]]> Exogenous gonadotrophin stimulation induces partial maturation of human sertoli cells in a testicular xenotransplantation model for fertility preservation /manager/Repository/uon:45062 n = 9, 1 year-adult). Human fetal testis (n = 6; 14-21 gestational weeks) tissue, which models many aspects of prepubertal testicular development, was transplanted subcutaneously into castrated immunocompromised mice for ~12 months. The mice received exogenous human chorionic gonadotropin (hCG; 20IU, 3x/week). In xenografts exposed continuously to hCG, we demonstrate the maintenance of Leydig cell steroidogenesis, the acquisition of features of Sertoli cell maturation (androgen receptor, lumen development), and the formation of the blood-testis barrier (connexin 43), none of which were present prior to the transplantation or in xenografts in which hCG was withdrawn after 7 months. These studies provide evidence that hCG plays a role in Sertoli cell maturation, which is relevant for future investigations, helping them generate functional gametes from immature testis tissue for clinical application.]]> Wed 26 Oct 2022 12:10:47 AEDT ]]> Sertoli cells as key drivers of testis function /manager/Repository/uon:44770 Mon 24 Oct 2022 09:03:28 AEDT ]]>