https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Folate and microRNA: bidirectional interactions https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30119 Wed 04 Sep 2019 09:38:53 AEST ]]> Circulating markers to assess nutritional therapy in cystic fibrosis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:660 Thu 25 Jul 2013 09:10:27 AEST ]]> Targeted arginine metabolomics: a rapid, simple UPLC-QToF-MS<sup>E</sup> based approach for assessing the involvement of arginine metabolism in human disease https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28420 G-monomethyl-L-arginine (MMA), NG,. NG-dimethyl-L-arginine (ADMA) and NG,. NG'-dimethyl-L-arginine (SDMA), all of which have the capacity to alter NOS activity. Simultaneous assessment of these analytes, when assessing the impact of arginine metabolism in human disease states, is desirable. Methods: Analytes (ARG, ADMA, SDMA, MMA, HMA, CIT and ORN) were isolated from human plasma by solvent extraction, evaporated and reconstituted. Ultra-performance liquid chromatography (UPLC) was performed on a 150mm×2.1mm T3 HSS column using a gradient mobile phase comprising ammonium formate (10mM, pH3.8) in methanol (1% to 63%). Analytes were detected by time-of-flight mass spectrometry (Q-ToF-MS) in positive ion mode with electrospray ionisation (ESI+). Data were collected using MSE. Results: Solvent extraction provided high recovery (>95%). UPLC-QToF-MSE facilitated the separation and quantification of the 7 analytes in an analysis time of 6min. The approach has high sensitivity; LOQ range from 0.005µM (NMMA) to 0.25µM (ARG and ORN), and good precision; intra- and inter-day %RSD are <6% for all analytes. Conclusions: This approach provides the capacity to quantify 7 key compounds involved in ARG metabolism in a small sample volume, with a short total analysis time. These characteristics make this approach ideal for undertaking a comprehensive characterisation of this pathway in large data sets (e.g. population studies).]]> Sat 24 Mar 2018 07:29:06 AEDT ]]> A novel missense LIPA gene mutation, N98S, in a patient with cholesteryl ester storage disease https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:4785 Sat 24 Mar 2018 07:20:44 AEDT ]]> Assessing vitamin D nutritional status: is capillary blood adequate? https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24470 Sat 24 Mar 2018 07:17:21 AEDT ]]>