https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Ovarian hormones through Wnt signalling regulate the growth of human and mouse ovarian cancer initiating lesions https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29580 BRCA1/2 mutations have a genetic predisposition for developing OC, but not all of these women develop the disease. Epidemiological findings show that lifestyle factors such as contraceptive use and pregnancy, a progesterone dominant state, decrease the risk of getting OC. How ovarian hormones modify the risk of OC is currently unclear. Our study identifies activated Wnt signalling to be a marker for precursor lesions of OC and successfully develops a mouse model that mimics the earliest events in pathogenesis of OC by constitutively activating ßcatenin. Using this model and human OC cells, we show that oestrogen promotes and progesterone suppresses the growth of OC cells.]]> Wed 11 Apr 2018 17:04:27 AEST ]]> Inhibition of extracellular matrix mediated TGF-β signalling suppresses endometrial cancer metastasis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30784 Wed 11 Apr 2018 13:21:37 AEST ]]> Overactive mTOR signaling leads to endometrial hyperplasia in aged women and mice https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30999 Wed 11 Apr 2018 09:51:53 AEST ]]> Proteomic analysis of stromal and epithelial cell communications in human endometrial cancer using a unique 3D co-culture model https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36541 Mon 01 Jun 2020 12:52:51 AEST ]]> Proteomic profiling of human uterine fibroids reveals upregulation of the extracellular matrix protein periostin https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35929 MED12 alterations in 39 of 65 fibroids (60%) from 14 patients. Using isobaric tagged-based quantitative mass spectrometry on three selected patients (n = 9 fibroids), we observed a common set of upregulated ( > 1.5-fold) and downregulated ( < 0.66-fold) proteins in small, medium, and large fibroid samples of annotated MED12 status. These two sets of upregulated and downregulated proteins were the same in all patients, regardless of variations in fibroid size and MED12 status. We then focused on one of the significant upregulated ECM proteins and confirmed the differential expression of periostin using western blotting and immunohistochemical analysis. Our study defined the proteome of uterine fibroids and identified that increased ECM protein expression, in particular periostin, is a hallmark of uterine fibroids regardless of MED12 mutation status. This study sets the foundation for further investigations to analyze the mechanisms regulating ECM overexpression and the functional role of upregulated ECM proteins in leiomyogenesis.]]> Fri 17 Jan 2020 11:35:08 AEDT ]]>