/manager/Index ${session.getAttribute("locale")} 5 Dissociation and preservation of preantral follicles and immature oocytes from female dasyurid marsupials /manager/Repository/uon:7747 Sat 24 Mar 2018 08:41:50 AEDT ]]> Comparison of the production, quality, and in vitro maturation capacity of oocytes from untreated cycling and intermediate phase equine serum gonadotropin-treated fat-tailed dunnarts (Sminthopsis crassicaudata) /manager/Repository/uon:7794 12 month) fat-tailed dunnarts, Sminthopsis crassicaudata. Increased urinary cornified epithelial cells and the influx of leukocytes defined day 0, at which time the naturally cycling females had already ovulated; at day 16 females had no antral follicles, but by day 20 antral follicles had begun to develop. There was no difference between naturally cycling breeding and retired females. Females were stimulated with 1 IU equine serum gonadotropin (eSG) during the intermediate phase on day 16 and killed 3, 4, or 5 days later. Stimulation resulted in a significant increase in the number of growing antral follicles but retired females demonstrated a reduced response. Upon collection from breeding females 4 days following eSG stimulation, 100% of oocytes were at the first polar body (PB1) stage, those collected from retired females were immature upon collection but within 48 h 98.2±1.9% were cultured to the PB1 stage. The rate of ovulation was high in breeding females 5 days following stimulation but retired females were less reliable, and in both groups all oocytes were degraded. This is the first study to describe a reliable technique, involving ovarian stimulation during the intermediate phase and segregation of age groups, allowing the collection of a large number of healthy PB1 stage oocytes from S. crassicaudata. This is important for the development of further assisted reproductive techniques for this species and threatened dasyurids.]]> Sat 24 Mar 2018 08:39:18 AEDT ]]> Acrosomal integrity, viability, and DNA damage of sperm from dasyurid marsupials after freezing or freeze drying /manager/Repository/uon:8081 Sat 24 Mar 2018 08:34:26 AEDT ]]> Development and evaluation of electroejaculation techniques in the Tasmanian devil (Sarcophilus harrisii) /manager/Repository/uon:19213 0.05). Increases observed in serum cortisol concentrations during EEJ were less (P<0.05) than those observed after an adrenalcorticotropic hormone challenge and diurnal variation suggested that cortisol concentrations are greater during the day than at night (P<0.05). This information can be used to provide range values for the future examination of basic endocrine responses and the adrenal-pituitary axis of this species. This study also demonstrated that spermatozoa-rich devil electroejaculates are more difficult to obtain and poorer in quality than those of other marsupials.]]> Sat 24 Mar 2018 07:54:58 AEDT ]]>