- Title
- Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting
- Creator
- Gangeswaran, Rathi; Jones, Keith T.
- Relation
- FEBS Letters Vol. 412, Issue 2, p. 309-312
- Publisher Link
- http://dx.doi.org/10.1016/S0014-5793(97)00782-5
- Publisher
- Elsevier BV
- Resource Type
- journal article
- Date
- 1997
- Description
- rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-δ and -λ were detected while such techniques failed to observe the conventional isoforms of PKC-α, -β, -γ. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of PCR products derived from conventional and novel PKC isoforms. In addition PKC-ε, -η, -θ and -ζ were not detected by rtPCR. These results suggest that the predominant isoforms in oocytes are PKC-δ and -λ.
- Subject
- protein kinase C; oocytes; calcium; fertilization; mouse; PCR
- Identifier
- uon:6586
- Identifier
- http://hdl.handle.net/1959.13/804311
- Identifier
- ISSN:0014-5793
- Language
- eng
- Reviewed
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