A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca²⁺ release in sea urchin egg homogenates

Title: A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca²⁺ release in sea urchin egg homogenates
Creator: Jones, Keith T.; Cruttwell, Caroline; Parrington, John; Swann, Swann
Relation: FEBS Letters Vol. 437, Issue 3, p. 297-300
Publisher Link: http://dx.doi.org/10.1016/S0014-5793(98)01254-X
Publisher: Elsevier BV
Resource Type: journal article
Date: 1998
Description: Injection of sperm extracts triggers Ca²⁺ oscillations in mammalian eggs similar to those seen at fertilisation. Here, we show that addition of sperm extracts to sea urchin egg homogenates causes Ca²⁺ release and inositol 1,4,5-trisphosphate (InsP₃) production. Furthermore depleting homogenates of phosphatidylinositol lipids using a phosphatidylinositol-specific phospholipase C blocked the sperm extract from causing InsP₃ production and a Ca²⁺ rise. A response could be recovered by the addition of phosphatidylinositol 4,5-bisphosphate to either sperm extracts or egg homogenates. These data indicate that sperm extracts contain an InsP₃-generating phospholipase C which may play a role in Ca²⁺ release at fertilisation.
Subject: egg; sperm; calcium; inositol trisphosphate; phospholipase C
Identifier: uon:6582
Identifier: http://hdl.handle.net/1959.13/804299
Identifier: ISSN:0014-5793
Language: eng
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