- Title
- A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca²⁺ release in sea urchin egg homogenates
- Creator
- Jones, Keith T.; Cruttwell, Caroline; Parrington, John; Swann, Swann
- Relation
- FEBS Letters Vol. 437, Issue 3, p. 297-300
- Publisher Link
- http://dx.doi.org/10.1016/S0014-5793(98)01254-X
- Publisher
- Elsevier BV
- Resource Type
- journal article
- Date
- 1998
- Description
- Injection of sperm extracts triggers Ca²⁺ oscillations in mammalian eggs similar to those seen at fertilisation. Here, we show that addition of sperm extracts to sea urchin egg homogenates causes Ca²⁺ release and inositol 1,4,5-trisphosphate (InsP₃) production. Furthermore depleting homogenates of phosphatidylinositol lipids using a phosphatidylinositol-specific phospholipase C blocked the sperm extract from causing InsP₃ production and a Ca²⁺ rise. A response could be recovered by the addition of phosphatidylinositol 4,5-bisphosphate to either sperm extracts or egg homogenates. These data indicate that sperm extracts contain an InsP₃-generating phospholipase C which may play a role in Ca²⁺ release at fertilisation.
- Subject
- egg; sperm; calcium; inositol trisphosphate; phospholipase C
- Identifier
- uon:6582
- Identifier
- http://hdl.handle.net/1959.13/804299
- Identifier
- ISSN:0014-5793
- Language
- eng
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