The soluble sperm factor that causes Ca²⁺ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca²⁺ oscillations after injection into mouse eggs

Title: The soluble sperm factor that causes Ca²⁺ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca²⁺ oscillations after injection into mouse eggs
Creator: Parrington, John; Jones, Keith T.; Lai, F. Anthony; Swann, Karl
Relation: Biochemical Journal Vol. 341, Issue 1, p. 1-4
Relation: http://www.biochemj.org/bj/341/bj3410001.htm
Publisher: Portland Press
Resource Type: journal article
Date: 1999
Description: Cytosolic extracts of boar sperm contain a soluble phospholipase C (PLC) activity that induces Ca²⁺ release in sea-urchin (Lytechinus pictus) egg homogenates and an uncharacterized protein factor that causes Ca²⁺ oscillations when injected into mammalian eggs. In the present study we fractionated boar sperm extracts on three different FPLC chromatographic columns and found that the fractions that caused maximal Ca²⁺ release in sea-urchin egg homogenates were also the ones that triggered Ca²⁺ oscillations in mouse eggs. Our data suggests that the sperm factor which triggers Ca²⁺ oscillations in eggs contains a PLC and not the 33 kDa glucosamine deaminase previously suggested to be one its components.
Subject: cytosolic; phospholipase C; calcium; egg homogenates; sperm; oscillations
Identifier: uon:6581
Identifier: http://hdl.handle.net/1959.13/804296
Identifier: ISSN:0264-6021
Language: eng
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