- Title
- Ca²⁺ oscillations promote APC/C-dependent cyclin B1 degradation during metaphase arrest and completion of meiosis in fertilizing mouse eggs
- Creator
- Nixon, Victoria L.; Levasseur, Mark; McDougall, Alex; Jones, Keith T.
- Relation
- Current Biology Vol. 12, Issue 9, p. 746-750
- Publisher Link
- http://dx.doi.org/10.1016/S0960-9822(02)00811-4
- Publisher
- Cell Press
- Resource Type
- journal article
- Date
- 2002
- Description
- Cyclin B1, the regulatory component of M phase-promoting factor (MPF), is degraded during the metaphase-anaphase transition in an anaphase-promoting complex/cyclosome (APC/C)-dependent process MPF activity is stable in eggs, and a sperm-triggered Ca²⁺ signal is needed to promote cyclin degradation. In frogs, a single Ca²⁺ spike promotes cell cycle resumption, but, in mammals, the Ca²⁺ signal is more complex, consisting of a series of spikes that stop several hours after sperm fusion. Using dual imaging in mouse eggs, we have examined how the Ca²⁺ signal generates cyclin B1 destruction using destructible and nondestructible GFP-tagged constructs. APC/C activity was present in unfertilized eggs, giving cyclin B1 a half-life of 1.15 ± 0.28 hr. However, APC/C-dependent cyclin degradation was elevated 6-fold when sperm raised cytosolic Ca²⁺ levels above 600 nM. This activation was transitory since cyclin B1 levels recovered between Ca²⁺ spikes. For continued cyclin degradation at basal Ca²⁺ levels, multiple spikes were needed. APC/C-mediated degradation was observed until eggs had completed meiosis with the formation of pronuclei, and, at this time, Ca²⁺ spikes stopped. Therefore, the physiological need for a repetitive Ca²⁺ signal in mammals is to ensure long-term cyclin destruction during a protracted exit from meiosis.
- Subject
- mouse; sperm; mammals; cyclin; degradation; eggs; meiosis; metaphase
- Identifier
- uon:6570
- Identifier
- http://hdl.handle.net/1959.13/804266
- Identifier
- ISSN:0960-9822
- Language
- eng
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