- Title
- Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes
- Creator
- Gorr, Ingo H.; Reis, Alexandra; Boos, Dominik; Wühr, Martin; Madgwick, Suzanne; Jones, Keith T.; Stemmann, Olaf
- Relation
- Nature Cell Biology Vol. 8, Issue 9, p. 1035-1037
- Publisher Link
- http://dx.doi.org/10.1038/ncb1467
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2006
- Description
- Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase–Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.
- Subject
- cdk1; meiosis; oocytes; anaphase; proteolytic; chromosome
- Identifier
- uon:6562
- Identifier
- http://hdl.handle.net/1959.13/804239
- Identifier
- ISSN:1465-7392
- Language
- eng
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