Lateral lamina V projection neuron axon collaterals connect sensory processing across the dorsal horn of the mouse spinal cord

Browne, Tyler J.; Smith, Kelly M.; Gradwell, Mark A.; Dayas, Christopher V.; Callister, Robert J.; Hughes, David I.; Graham, Brett A.

Title: Lateral lamina V projection neuron axon collaterals connect sensory processing across the dorsal horn of the mouse spinal cord
Creator: Browne, Tyler J.; Smith, Kelly M.; Gradwell, Mark A.; Dayas, Christopher V.; Callister, Robert J.; Hughes, David I.; Graham, Brett A.
Relation: NHMRC.631000 https://purl.org/au-research/grants/nhmrc/631000 & 1043933 https://purl.org/au-research/grants/nhmrc/1043933 & 1144638 https://purl.org/au-research/grants/nhmrc/1144638
Relation: Scientific Reports Vol. 14, no. 26354
Publisher Link: http://dx.doi.org/10.1038/s41598-024-73620-4
Publisher: Nature Publishing Group
Resource Type: journal article
Date: 2024
Description: Spinal projection neurons (PNs) are defined by long axons that travel from their origin in the spinal cord to the brain where they relay sensory information from the body. The existence and function of a substantial axon collateral network, also arising from PNs and remaining within the spinal cord, is less well appreciated. Here we use a retrograde viral transduction strategy to characterise a novel subpopulation of deep dorsal horn spinoparabrachial neurons. Brainbow assisted analysis confirmed that virally labelled PN cell bodies formed a discrete cell column in the lateral part of Lamina V (LVlat) and the adjoining white matter. These PNs exhibited large dendritic territories biased to regions lateral and ventral to the cell body column and extending considerable rostrocaudal distances. Optogenetic activation of LVLat PNs confirmed this population mediates widespread signalling within spinal cord circuits, including activation in the superficial dorsal horn. This signalling was also demonstrated with patch clamp recordings during LVLat PN photostimulation, with a range of direct and indirect connections identified and evidence of a postsynaptic population of inhibitory interneurons. Together, these findings confirm a substantial role for PNs in local spinal sensory processing, as well as relay of sensory signals to the brain.
Subject: projection neurons (PNs); spinal cord; sensory information; cell bodies
Identifier: http://hdl.handle.net/1959.13/1517467
Identifier: uon:57114
Identifier: ISSN:2045-2322
Rights: x
Language: eng
Reviewed

Hits: 184
Visitors: 184
Downloads: 0