- Title
- The role of the tetraspanin CD151 in primary keratinocyte and fibroblast functions: implications for wound healing
- Creator
- Geary, Sean M.; Cowin, Allison J.; Copeland, Ben; Baleato, Rosa M.; Miyazaki, Kaoru; Ashman, Leonie K.
- Relation
- Experimental Cell Research Vol. 314, Issue 11-12, p. 2165-2175
- Publisher Link
- http://dx.doi.org/10.1016/j.yexcr.2008.04.011
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2008
- Description
- Previous studies showed that CD151-null mice have a skin wound healing deficit. To gain an understanding of the role of CD151 in re-epithelialisation and dermal contraction, keratinocyte and fibroblast functions were assayed. Primary CD151-null keratinocytes displayed defective migration on Matrigel (a basement membrane equivalent) and laminin-332, the primary adhesion component of basement membranes, but not on collagen-I. Adhesion, spreading and proliferation were also deficient on laminin-332, but not collagen-I. The data suggest that loss of CD151 impairs the function of its primary interaction partners, integrin α3β1- and/or α6β4 which bind to laminin-332. Skin fibroblasts also produce CD151 mRNA. CD151-null fibroblasts migrated significantly faster on collagen I than wild type fibroblasts, confirming that they possess functional collagen receptors. However, no significant decrease in the ability of CD151-null fibroblasts to cause contraction in floating collagen gel assays in response to transforming growth factor beta-1 (TGF-β1) or platelet derived growth factor (PDGF-BB) was observed, nor was there an effect on fibroblast adhesion or proliferation on collagen-I. The data implicate CD151 as a facilitator of laminin-332-mediated keratinocyte functions that impact on the re-epithelialisation process intrinsic to wound healing and further suggest a potential novel role for CD151 in fibroblast migration.
- Subject
- CD151; fibroblast; keratinocyte; laminin-332; re-epithelialization; skin wound healing; tetraspanin
- Identifier
- uon:5569
- Identifier
- http://hdl.handle.net/1959.13/43456
- Identifier
- ISSN:0014-4827
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