- Title
- Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor
- Creator
- Yousefi, Meisam; Lee, Wai Suet; Chan, Wharton O. Y.; He, Wei; Mah, Marcus G.; Yong, Cythia Lingli; Deerain, Joshua M.; Wang, Lijin; Arcinas, Camille; Yan, Biaoguo; Tan, Dewei; Sia, Wan Rong; Gamage, Akshamal M.; Yang, Jinxuan; Hsu, Alan Chen-Yu; Li, Shang; Linster, Martin; Yang, Xinglou; Ghosh, Sujoy; Anderson, Danielle E.
- Relation
- Emerging Microbes & Infections Vol. 12, Issue 2, no. 2256416
- Publisher Link
- http://dx.doi.org/10.1080/22221751.2023.2256416
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2023
- Description
- The emergence of novel betacoronaviruses has posed significant financial and human health burdens, necessitating the development of appropriate tools to combat future outbreaks. In this study, we have characterized a human cell line, IGROV-1, as a robust tool to detect, propagate, and titrate betacoronaviruses SARS-CoV-2 and HCoV-OC43. IGROV-1 cells can be used for serological assays, antiviral drug testing, and isolating SARS-CoV-2 variants from patient samples. Using time-course transcriptomics, we confirmed that IGROV-1 cells exhibit a robust innate immune response upon SARS-CoV-2 infection, recapitulating the response previously observed in primary human nasal epithelial cells. We performed genome-wide CRISPR knockout genetic screens in IGROV-1 cells and identified Aryl hydrocarbon receptor (AHR) as a critical host dependency factor for both SARS-CoV-2 and HCoV-OC43. Using DiMNF, a small molecule inhibitor of AHR, we observed that the drug selectively inhibits HCoV-OC43 infection but not SARS-CoV-2. Transcriptomic analysis in primary normal human bronchial epithelial cells revealed that DiMNF blocks HCoV-OC43 infection via basal activation of innate immune responses. Our findings highlight the potential of IGROV-1 cells as a valuable diagnostic and research tool to combat betacoronavirus diseases.
- Subject
- igrov-1; covid-19; betacoronavirus; sars-cov-2; hcov-oc43; Genome-scale CRISPR screening; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1500046
- Identifier
- uon:54840
- Rights
- © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
- Language
- eng
- Full Text
- Reviewed
- Hits: 3633
- Visitors: 3657
- Downloads: 44
Collections
Goal 3: Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 4 MB | Adobe Acrobat PDF | View Details Download |