- Title
- Bio-maleimide as a generic stain for detection and quantitation of microparticles
- Creator
- Enjeti, A. K.; Lincz, L.; Seldon, M.
- Relation
- International Journal of Laboratory Hematology Vol. 30, Issue 3, p. 196-199
- Publisher Link
- http://dx.doi.org/10.1111/j.1751-553X.2007.00937.x
- Publisher
- Blackwell Publishing
- Resource Type
- journal article
- Date
- 2008
- Description
- Microparticles (MP) are small fragments of cytoplasm shed from a cell surface and their role in the pathophysiology of disease is being extensively investigated. A novel staining technique for quantifying total MP in peripheral blood was evaluated in this study. Evaluation of Bodipy-maleimide (or bio-maleimide) as a stain for quantifying total MP in peripheral blood by flow cytometry. Samples were obtained from 10 healthy donors after informed consent. Plasma was prepared by sequential centrifugation at 1500 ℊ followed by 13 000 ℊ and stained with Annexin V and bio-maleimide. Enumeration beads were added after 15 min of incubation with the stain and samples analyzed on a FACS Canto flow cytometer. Detection and quantification of MP by bio-maleimide staining was comparable with that by Annexin V. The total mean MP level with bio-maleimide staining was 34 ± 19.7/μl (range of 11.6–68.1/μl) and with Annexin V staining it was 38.9 ± 29.8/μl (range of 10.6 to 112.9/μl). There was no significant difference using a paired t-test and methods were comparable using a Bland–Altman plot. Bio-maleimide is a useful and inexpensive stain to measure total MP levels in peripheral blood by flow cytometry. This technique could be employed to study thrombotic risks in a variety of disease states.
- Subject
- microparticles; thrombosis; maleimide; quantitation; analytical variables
- Identifier
- http://hdl.handle.net/1959.13/43244
- Identifier
- uon:5365
- Identifier
- ISSN:1751-5521
- Language
- eng
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