- Title
- Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice
- Creator
- Thorpe, Andrew E.; Donovan, Chantal; Kim, Richard Y.; Vindin, Howard J.; Zakarya, Razia; Miyai, Hanna; Chan, Yik L.; van Reyk, David; Chen, Hui; Oliver, Brian G.
- Relation
- Toxics Vol. 11, Issue 9, no. 749
- Publisher Link
- http://dx.doi.org/10.3390/toxics11090749
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Resource Type
- journal article
- Date
- 2023
- Description
- In the last decade, e-cigarette usage has increased, with an estimated 82 million e-cigarette users globally. This is, in part, due to the common opinion that they are “healthier” than tobacco cigarettes or simply “water vapour”. Third-hand e-vapour exposure is the chemical residue left behind from e-cigarette aerosols, which is of concern due to its invisible nature, especially among young children. However, there is limited information surrounding third-hand e-vapour exposure. This study aimed to investigate the pulmonary effects of sub-chronic third-hand e-vapour exposure in a murine model. BALB/c mice (4 weeks of age) were exposed to a towel containing nicotine free (0 mg) e-vapour, nicotine (18 mg) e-vapour, or no e-vapour (sham) and replaced daily for 4 weeks. At the endpoint, lung function was assessed, and bronchoalveolar lavage fluid and lungs were collected to measure inflammation and fibrosis. Mice exposed to third-hand e-vapour without nicotine had alveolar enlargement compared to sham exposed controls. Mice exposed to third-hand e-vapour with nicotine had reduced bronchial responsiveness to provocation, increased epithelial thickening in large airways, increased epithelial layers in small airways, alveolar enlargement, and increased small airway collagen deposition, compared to sham exposed controls. In conclusion, our study shows that third-hand e-vapour exposure, particularly in the presence of nicotine, negatively affects the lung health of mice and highlights the need for greater public awareness surrounding the dangers of third-hand exposure to e-cigarette vapour.
- Subject
- e-cigarettes; vaping; e-vaping; nicotine; lung function; fibrosis
- Identifier
- http://hdl.handle.net/1959.13/1492937
- Identifier
- uon:53453
- Identifier
- ISSN:2305-6304
- Rights
- © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Language
- eng
- Full Text
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