- Title
- Non-Lethal Sampling Does Not Misrepresent Trophic Level or Dietary Sources for Sagmariasus Verreauxi (Eastern Rock Lobster)
- Creator
- Day, Jeremy Karl; Knott, Nathan Aaron; Swadling, Daniel; Ayre, David; Huggett, Megan; Gaston, Troy
- Relation
- Rapid Communications in Mass Spectrometry Vol. 37, Issue 4, no. e9435
- Publisher Link
- http://dx.doi.org/10.1002/rcm.9435
- Publisher
- John Wiley & Sons
- Resource Type
- journal article
- Date
- 2023
- Description
- Rationale: Isotope analysis can be used to investigate the diets of predators based on assimilation of nitrogen and carbon isotopes from prey. Recent work has shown that tissues taken from legs, antennae or abdomen of lobsters can give different indications of diet, but this has never been evaluated for Sagmariasus verreauxi (eastern rock lobster). Work is now needed to prevent erroneous conclusions being drawn about lobster food webs, and undertaking this work could lead to developing non-lethal sampling methodologies. Non-lethal sampling for lobsters is valuable both ethically and for areas of conservation significance such as marine reserves. Method: We evaluated this by dissecting 76 lobsters and comparing δ13C and δ15N isotope values in antennae, leg and abdomen tissue from the same individuals ranging from 104 to 137 mm carapace length. Stable isotope values were determined using a Europa EA GSL elemental analyser coupled with Hydra 20–20 Isoprime IRMS.Results: We found the abdomen δ13C values to be lower than other tissues by 0.3 ± 0.2‰ for antennae tissue and 0.1 ± 0.2‰ δ13C for leg tissues, whereas for δ15N, no significant difference between tissues was observed. There was no significant effect of lobster size or sex, though we did observe interactions between month and tissue type, indicating that differences may be seasonal. Importantly, the detected range of isotopic variability between tissues is within the range of uncertainty used for discrimination factors in isotopic Bayesian modelling of 0‰–1.0‰ for δ13C and 3.0‰–4.0‰ for δ15N. Conclusions: We show that S. verreauxi can be sampled non-lethally with mathematical corrections applied for δ13C, whereas any tissue is suitable for δ15N. Our results indicate that a walking leg is most favourable and would also be the least intrusive for the lobster. The application of non-lethal sampling provides avenues for the contribution of citizen science to understanding lobster food webs and to undertake fieldwork in ecologically sensitive areas such as marine reserves.
- Subject
- Sagmariasus verreauxi (eastern rock lobster); diet; lobster food webs; marine reserves; SDG 14; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1480869
- Identifier
- uon:50578
- Identifier
- ISSN:0951-4198
- Rights
- © 2022 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. (http://creativecommons.org/licenses/by/4.0/).
- Language
- eng
- Full Text
- Reviewed
- Hits: 1583
- Visitors: 1638
- Downloads: 68
Collections
Goal 14: Life Below Water
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 1011 KB | Adobe Acrobat PDF | View Details Download |