- Title
- A stallion spermatozoon's journey through the mare's genital tract: In vivo and in vitro aspects of sperm capacitation
- Creator
- Maitan, Paula; Bromfield, Elizabeth G.; Stout, Tom A. E.; Gadella, Bart M.; Leemans, Bart
- Relation
- Animal Reproduction Science Vol. 246, Issue November 2022, no. 106848
- Publisher Link
- http://dx.doi.org/10.1016/j.anireprosci.2021.106848
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2022
- Description
- Conventional in vitro fertilization is not efficacious when working with equine gametes. Although stallion spermatozoa bind to the zona pellucida in vitro, these gametes fail to initiate the acrosome reaction in the vicinity of the oocyte and cannot, therefore, penetrate into the perivitelline space. Failure of sperm penetration most likely relates to the absence of optimized in vitro fertilization media containing molecules essential to support stallion sperm capacitation. In vivo, the female reproductive tract, especially the oviductal lumen, provides an environmental milieu that appropriately regulates interactions between the gametes and promotes fertilization. Identifying these ‘fertilization supporting factors’ would be a great contribution for development of equine in vitro fertilization media. In this review, a description of the current understanding of the interactions stallion spermatozoa undergo during passage through the female genital tract, and related specific molecular changes that occur at the sperm plasma membrane is provided. Understanding these molecular changes may hold essential clues to achieving successful in vitro fertilization with equine gametes.
- Subject
- oviduct; horse; sperm capacitation; membrane changes
- Identifier
- http://hdl.handle.net/1959.13/1478311
- Identifier
- uon:50152
- Identifier
- ISSN:0378-4320
- Rights
- © 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Language
- eng
- Full Text
- Reviewed
- Hits: 676
- Visitors: 717
- Downloads: 45
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 2 MB | Adobe Acrobat PDF | View Details Download |