- Title
- T lymphocyte and monocyte subsets are dysregulated in type 1 diabetes patients with peripheral neuropathic pain
- Creator
- O'Brien, Jayden A.; McGuire, Helen M.; Shinko, Diana; Fazekas de St Groth, Barbara; Russo, Marc A.; Bailey, Dominic; Santarelli, Danielle M.; Wynne, Katie; Austin, Paul J.
- Relation
- Brain, Behavior, and Immunity - Health Vol. 15, Issue August 2021, no. 100283
- Publisher Link
- http://dx.doi.org/10.1016/j.bbih.2021.100283
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2021
- Description
- Diabetic neuropathic pain is a common and devastating complication of type 1 diabetes, but the mechanism by which it develops and persists is yet to be fully elucidated. This study utilised high-dimensional suspension mass cytometry in a pilot cohort to investigate differences in peripheral blood immunophenotypes between type 1 diabetes patients with (n = 9) and without (n = 9) peripheral neuropathic pain. The abundance and activation of several leukocyte subsets were investigated with unsupervised clustering approaches FlowSOM and SPADE, as well as by manual gating. Major findings included a proportional increase in CD4+ central memory T cells and an absolute increase in classical monocytes, non-classical monocytes, and mature natural killer cells in type 1 diabetes patients with pain compared to those without pain. The expression of CD27, CD127, and CD39 was upregulated on select T cell populations, and the phosphorylated form of pro-inflammatory transcription factor MK2 was upregulated across most populations. These results provide evidence that distinct immunological signatures are associated with painful neuropathy in type 1 diabetes patients. Further research may link these changes to mechanisms by which pain in type 1 diabetes is initiated and maintained, paving the way for much needed targeted treatments.
- Subject
- CD27; chronic pain; diabetic neuropathy; flowSOM; immunophenotyping; MAPKAPK2; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1476411
- Identifier
- uon:49817
- Identifier
- ISSN:2666-3546
- Rights
- © 2021 The Authors. Published by Elsevier Inc.. This is an open access article under the CC BY-NC-ND license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Language
- eng
- Full Text
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