- Title
- Algalization of Acid Soils with Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3 Enriches Bacteria of Ecological Importance
- Creator
- Abinandan, Sudharsanam; Shanthakumar, Subramanian; Panneerselvan, Logeshwaran; Venkateswarlu, Kadiyala; Megharaj, Mallavarapu
- Relation
- ACS Agricultural Science and Technology Vol. 2, Issue 3, p. 512-520
- Publisher Link
- http://dx.doi.org/10.1021/acsagscitech.1c00277
- Publisher
- American Chemical Society
- Resource Type
- journal article
- Date
- 2022
- Description
- Acid soils are the degraded (nutrient-poor) soils that generally lack microbial abundance required to promote plant growth. An insight into the microbial diversity in highly acidic soils is crucial from both ecological and environmental standpoints. Previously, we showed that inoculation of acid soils with acid-tolerant microalgae (algalization) significantly improved soil physicochemical and biological characteristics. In the present novel study involving a laboratory microcosm, high-throughput 16S rRNA amplicon sequencing analysis was performed to investigate the bacterial diversity in acid soils algalized with Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3 after 90 days of incubation. Our results on pooled DNA demonstrate that algalization of two acid soils (soil A and B) significantly increased several bacterial genera, and this observation is consistent with Shannon and Chao1 diversity indices. Actinobacteria, Acidobacteria, Firmicutes, and Proteobacteria were the most prevalent phyla enriched in all of the algalized treatments. Interestingly, nonalgalized acid soils favored only Firmicutes and Actinobacteria, but algalization significantly enriched Proteobacteria, Acidobacteria, and Actinobacteria. Canonical correspondence analysis revealed a positive effect of pH in soil A and both pH and organic carbon in soil B on enrichment. Furthermore, soil bacteria of ecological significance that belong to rhizobacteria and diazotrophs, such as Acetobacter, Azospirillum, Bradyrhizobium, Gluconacetobacter, Nitrobacter, Burkholderia, Comamonas, Herbaspirillum, Enterobacter, Nitrosococcus, Brevibacillus, Enterococcus, Frankia, and Anabaena, were greatly enriched in algalized treatments. Thus, we demonstrate here for the first time that algalization of acid soils significantly improves soil health through enrichment of bacteria that are largely implicated in promoting soil health and plant growth.
- Subject
- algalization; microalgae; soil health; pooled DNA; plant growth-promoting bacteria
- Identifier
- http://hdl.handle.net/1959.13/1470329
- Identifier
- uon:48429
- Identifier
- ISSN:2692-1952
- Language
- eng
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