- Title
- Molecular mechanisms underlying titanium dioxide nanoparticles (TiO2NP) induced autophagy in mesenchymal stem cells (MSC)
- Creator
- Yu, Shunbang; Mu, Yongping; Zhang, Xudong; Li, Jian; Lee, Charles; Wang, He
- Relation
- Journal of Toxicology and Environmental Health - Part A: Current Issues Vol. 82, Issue 18, p. 997-1008
- Publisher Link
- http://dx.doi.org/10.1080/15287394.2019.1688482
- Publisher
- Taylor & Francis
- Resource Type
- journal article
- Date
- 2019
- Description
- The bone marrow is one of the target tissues for titanium dioxide nanoparticles (TiO2NP) following environmental exposure. At present, the consequences of TiO2NP exposure in bone are not well known. The aim of this study was to investigate the effects of TiO2NP on mesenchymal stem cells (MSCs) and potential underlying mechanisms. Mesenchymal bone marrow-derived cells were cultured and treated with various concentrations of TiO2NP. Results showed that TiO2NP incubation produced cytotoxicity as evidenced by reduced cell viability. Using Western blotting TiO2NP was found to increase autophagy as determined by elevation in ratio of LC3-II from LC3-I without evidence of necrotic cell death as estimated by lactic dehydrogenase (LDH) level. TiO2NP produced a rise in intracellular reactive oxygen species (ROS) levels. The observed alterations in autophagy and oxidant stress were associated with upregulation of protein expression of p38, JNK, and ERK. Data indicate that TiO2NP-mediated decrease in MSC survival involves a complex series of events associated stimulation of mitogen-activated protein kinase (MAPK) pathway and consequent autophagy and oxidative damage.
- Subject
- titanium dioxide nanoparticles; autophagy; reactive oxygen species; mitogen-activated protein kinase; mesenchymal stem sells
- Identifier
- http://hdl.handle.net/1959.13/1463140
- Identifier
- uon:46649
- Identifier
- ISSN:1528-7394
- Language
- eng
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