- Title
- Dissimilatory nitrate reduction to ammonium increased with rising temperature
- Creator
- Lai, Thang V.; Ryder, Maarten H.; Rathjen, Judith R.; Bolan, Nanthi S.; Croxford, Adam E.; Denton, Matthew D.
- Relation
- Biology and Fertility of Soils Vol. 57, Issue 3, p. 363-372
- Publisher Link
- http://dx.doi.org/10.1007/s00374-020-01529-x
- Publisher
- Springer
- Resource Type
- journal article
- Date
- 2021
- Description
- Although recent studies have explored dissimilatory nitrate reduction to ammonium (DNRA), little is known about the dependence of this process on temperature. A laboratory experiment was conducted to investigate if temperature regulates nitrate (NO3ˉ) reduction by DNRA and denitrification. Soil samples were incubated at 10 to 40 °C for 28 days, and 15N tracer methods were applied to estimate the reduction of 15NO3ˉ to 15NH4+ by DNRA organisms and to 15N2O or 15N2 by denitrifiers. Raising the temperature from 10 to 40 °C increased DNRA, which contributed to enrichment of the NH4+ pool, particularly at 40 °C. The percentage of 15NO3ˉ, reduced by DNRA was from 4 to 30% of that reduced by denitrification, and decreased with increasing temperature. DNRA and denitrification were positively correlated with the abundance of nrfA and nosZ genes, respectively. The accumulation of 15NH4+ slowed in the final 12 h of sampling times, which may be due to a re-conversion of 15NH4+ to 15NO3ˉ by nitrification. High temperatures (30 to 40 °C) enhanced 15N2 production, which suggests that temperature plays an important role in regulating complete denitrification from NO3ˉ to N2.
- Subject
- nosZ; nrfA; partioning of NO3; recovery of 15NH4
- Identifier
- http://hdl.handle.net/1959.13/1461890
- Identifier
- uon:46327
- Identifier
- ISSN:0178-2762
- Language
- eng
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