- Title
- Contraction in human myometrium is associated with changes in small heat shock proteins
- Creator
- MacIntyre, David A.; Tyson, Elisa K.; Read, Mark; Smith, Roger; Yeo, George; Kwek, Kenneth; Chan, Eng-Cheng
- Relation
- Endocrinology Vol. 149, Issue 1, p. 245-252
- Publisher Link
- http://dx.doi.org/10.1210/en.2007-0662
- Publisher
- Endocrine Society
- Resource Type
- journal article
- Date
- 2008
- Description
- The myometrium undergoes substantial remodeling at the time of labor including rearrangement of the cellular contractile machinery. The regulation of this process in human myometrium at the time of labor is poorly defined, but evidence in other muscle types suggests modulation by small heat shock proteins (sHSP). The aim of this study was to investigate whether similar changes in sHSP occur in the myometrium at labor. Using a quantitative proteomic approach (two-dimensional difference gel electrophoresis), we found a 69% decrease in the sHSP αB-crystallin in the myometrium at labor plus multiple isoforms of HSP27. Immunoblotting using phosphospecific HSP27 antibodies (HSP27-serine15, -78, and -82) detected marked changes in HSP27 phosphorylation at labor. Although total HSP27 levels were unchanged, HSP27-Ser15 was 3-fold higher at labor. Coimmunoprecipitation studies showed that HSP27 coprecipitates with αB-crystallin and also smooth muscle α-actin. Coimmunofluorescence studies demonstrated a relocation of HSP27 from the perinuclear region to the actin cytoskeleton at labor. The functional significance of these changes was demonstrated in vitro where myometrial strips stimulated to contract with oxytocin exhibited increased HSP27-Ser15 phosphorylation. Our findings provide data consistent with a novel pathway regulating human myometrial contraction at labor and identify HSP27 and αB-crystallin as potential targets for future tocolytic design.
- Subject
- αB-crystallin; vascular smooth muscle; P38 map kinase; actin
- Identifier
- http://hdl.handle.net/1959.13/40359
- Identifier
- uon:4588
- Identifier
- ISSN:0013-7227
- Language
- eng
- Full Text
- Reviewed
- Hits: 2361
- Visitors: 3370
- Downloads: 534
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT01 | Publisher version (open access) | 940 KB | Adobe Acrobat PDF | View Details Download |