- Title
- Circulating microvesicles in snakebite patients with microangiopathy
- Creator
- Enjeti, Anoop K.; Lincz, Lisa F.; Seldon, Michael; Isbister, Geoffrey K.
- Relation
- NSW Health Pathology/Hunter Cancer Research Alliance (HCRA)/Calvary Mater Newcastle (CMN) translational research fellowship
- Relation
- RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS Vol. 3, Issue 1, p. 121-125
- Publisher Link
- http://dx.doi.org/10.1002/rth2.12164
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Resource Type
- journal article
- Date
- 2019
- Description
- Background Venom‐induced consumption coagulopathy is a common consequence of snake envenoming that can lead to life‐threatening hemorrhage, and is associated with microangiopathic hemolytic anemia (MAHA ), acute kidney injury and thrombocytopenia. The role of microvesicles (MV ) in snakebite patients has not been previously investigated. Objective To compare changes in subsets of circulating MV levels in snakebite patients with venom induced consumption coagulopathy and with or without microangiopathic hemolysis to those of healthy controls. Methods This study used samples from patients recruited to the Australian Snakebite Project (ASP ) with snake envenoming, including bites by brown snakes, tiger snakes, and taipans. Citrated blood from envenomed patients was collected, processed, and stored according to a national standardized protocol. Full blood count and coagulation parameters were measured as per routine clinical care and blood films were examined for evidence of hemolysis. Baseline coagulation parameters were measured on a Behring Coagulation System. Flow cytometry was performed to detect CD 41a (platelet), CD 62e (endothelial), and glycophorin (red cell) MV . The results were analyzed using BD software and appropriate statistical tools.Results and Conclusions The red cell MV in snakebite patients with MAHA (n = 13) were significantly higher than those without MAHA (n = 17) while there was no significant difference in platelet MV levels between the snakebite patients with and without MAHA . Interestingly, the endothelial MV were reduced in all snakebite patient samples compared to the control samples. Measuring red cell MV at presentation could be useful as a predictive marker for MAHA in patients with snakebites.
- Subject
- coagulopathy; extracellular vesicles; microangiopathy; microvesicles; snakebite; venom
- Identifier
- http://hdl.handle.net/1959.13/1455409
- Identifier
- uon:45084
- Identifier
- ISSN:2475-0379
- Rights
- © 2018 The Authors. Research and Practice in Thrombosis and Haemostasis published by Wiley Periodicals, Inc on behalf of International Society on Thrombosis and Haemostasis. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
- Language
- eng
- Full Text
- Reviewed
- Hits: 839
- Visitors: 917
- Downloads: 84
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 414 KB | Adobe Acrobat PDF | View Details Download |