- Title
- Comparison of two transmission electron microscopy methods to visualize drug-induced alterations of gram-negative bacterial morphology
- Creator
- Nguyen, Hang Thi; O'Donovan, Lisa A.; Venter, Henrietta; Russell, Cecilia C.; McCluskey, Adam; Page, Stephen W.; Trott, Darren J.; Ogunniyi, Abiodun D.
- Relation
- ARC.LP110200770 http://purl.org/au-research/grants/arc/LP110200770
- Relation
- Antibiotics Vol. 10, Issue 3, no. 10030307
- Publisher Link
- http://dx.doi.org/10.3390/antibiotics10030307
- Publisher
- MDPI AG
- Resource Type
- journal article
- Date
- 2021
- Description
- In this study, we optimized and compared different transmission electron microscopy (TEM) methods to visualize changes to Gram-negative bacterial morphology induced by treatment with a robenidine analogue (NCL195) and colistin combination. Aldehyde-fixed bacterial cells (untreated, treated with colistin or NCL195 + colistin) were prepared using conventional TEM methods and compared with ultrathin Tokuyasu cryo-sections. The results of this study indicate superiority of ultrathin cryo-sections in visualizing the membrane ultrastructure of Escherichia coli and Pseudomonas aeruginosa, with a clear delineation of the outer and inner membrane as well as the peptidoglycan layer. We suggest that the use of ultrathin cryo-sectioning can be used to better visualize and understand drug interaction mechanisms on the bacterial cell membrane.
- Subject
- transmission electron microscopy; bacterial cell wall; bacterial membrane; Gram-negative bacteria; colistin; drug interaction; Tokuyasu cryo-ultramicrotomy
- Identifier
- http://hdl.handle.net/1959.13/1455115
- Identifier
- uon:45054
- Identifier
- ISSN:2079-6382
- Rights
- © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
- Language
- eng
- Full Text
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