- Title
- The Jemalong 2HA line used for Medicago truncatula transformation: hormonology and epigenetics
- Creator
- Rose, Ray J.; Song, Youhong
- Relation
- The Model Legume Medicago truncatula p. 1170-1178
- Publisher Link
- http://dx.doi.org/10.1002/9781119409144.ch151
- Publisher
- John Wiley & Sons Inc.
- Resource Type
- book chapter
- Date
- 2019
- Description
- The Medicago truncatula Jemalong 2HA line (2HA) was developed from continued selections from seeds of a cv. Jemalong plant, regenerated from tissue culture that was extremely embryogenic. While this genotype provides a resource for transformation, it also provides some generic insights into the hormonology of somatic embryogenesis, hormone interactions, epigenetics, and stress. In somatic embryogenesis of 2HA the exogenous phytohormones 1-naphthaleneacetic acid and 6-benzylamino purine are central to regeneration. However, there are interactions with ethylene, abscisic acid, and gibberellic acid, which produce maximum somatic embryogenesis. Not only are suitable interactions required for producing an embryogenic “stem” cell, appropriate hormone interaction is required to produce a fully developed embryo. Based on current information wild-type Jemalong and 2HA have similar genomic sequences, but methylation patterns are different. The 2HA line has inherited epigenetic change, notably the repression of MtEIL1 (ETHYLENE INSENSITIVE3 [EIN3]-like). This repression is associated with methylation of the coding region and an estimated 21 nt small RNA antisense to the 3′UTR. This is consistent with a weak ethylene insensitive phenotype, but it alone cannot explain the SE capacity. It is suggested that it was the stress of the cycle of tissue culture that produced the 2HA line. This is consistent with recent literature linking abiotic stress to epigenetic change which in some cases can be transgenerational and is further discussed in this chapter.
- Subject
- abiotic stress; DNA methylation; epigenetics; hormone interactions; jemalong 2HA; medicago truncatula
- Identifier
- http://hdl.handle.net/1959.13/1453180
- Identifier
- uon:44611
- Identifier
- ISBN:9781119409151
- Language
- eng
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