- Title
- Activity of two key toxin groups in Australian elapid venoms show a strong correlation to phylogeny but not to diet
- Creator
- Tasoulis, Theo; Lee, Michael S. Y.; Ziajko, Manon; Dunstan, Nathan; Sumner, Joanna; Isbister, Geoffrey K.
- Relation
- NHMRC.ID1061041 http://purl.org/au-research/grants/nhmrc/1061041
- Relation
- BMC Evolutionary Biology Vol. 20, Issue 1, no. 9
- Publisher Link
- http://dx.doi.org/10.1186/s12862-020-1578-x
- Publisher
- BioMed Central (BMC)
- Resource Type
- journal article
- Date
- 2020
- Description
- Background: The relative influence of diet and phylogeny on snake venom activity is a poorly understood aspect of snake venom evolution. We measured the activity of two enzyme toxin groups – phospholipase A2 (PLA2), and L-amino acid oxidase (LAAO) – in the venom of 39 species of Australian elapids (40% of terrestrial species diversity) and used linear parsimony and BayesTraits to investigate any correlation between enzyme activity and phylogeny or diet. Results: PLA2 activity ranged from 0 to 481 nmol/min/mg of venom, and LAAO activity ranged from 0 to 351 nmol/min/mg. Phylogenetic comparative methods, implemented in BayesTraits showed that enzyme activity was strongly correlated with phylogeny, more so for LAAO activity. For example, LAAO activity was absent in both the Vermicella and Pseudonaja/Oxyuranus clade, supporting previously proposed relationships among these disparate taxa. There was no association between broad dietary categories and either enzyme activity. There was strong evidence for faster initial rates of change over evolutionary time for LAAO (delta parameter mean 0.2), but no such pattern in PLA2 (delta parameter mean 0.64). There were some exceptions to the phylogenetic patterns of enzyme activity: different PLA2 activity in the ecologically similar sister-species Denisonia devisi and D. maculata; large interspecific differences in PLA2 activity in Hoplocephalus and Austrelaps. Conclusions: We have shown that phylogeny is a stronger influence on venom enzyme activity than diet for two of the four major enzyme families present in snake venoms. PLA2 and LAAO activities had contrasting evolutionary dynamics with the higher delta value for PLA2 Some species/individuals lacked activity in one protein family suggesting that the loss of single protein family may not incur a significant fitness cost.
- Subject
- snake; evolution; phylogeny; venom; snake venom; toxin; diet; phospholipase
- Identifier
- http://hdl.handle.net/1959.13/1452907
- Identifier
- uon:44536
- Identifier
- ISSN:1471-2148
- Rights
- © The Author(s). 2020. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Language
- eng
- Full Text
- Reviewed
- Hits: 2639
- Visitors: 2699
- Downloads: 70
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 1 MB | Adobe Acrobat PDF | View Details Download |