- Title
- Acid-adapted microalgae exhibit phenotypic changes for their survival in acid mine drainage samples
- Creator
- Abinandan, Sudharsanam; Perera, Isiri Adhiwarie; Subashchandrabose, Suresh R.; Venkateswarlu, Kadiyala; Cole, Nicole; Megharaj, Mallavarapu
- Relation
- FEMS Microbiology Ecology Vol. 96, Issue 11
- Publisher Link
- http://dx.doi.org/10.1093/femsec/fiaa113
- Publisher
- Oxford University Press
- Resource Type
- journal article
- Date
- 2020
- Description
- Phenotypic plasticity or genetic adaptation in an organism provides phenotypic changes when exposed to the extreme environmental conditions. The resultant physiological and metabolic changes greatly enhance the organism's potential for its survival in such harsh environments. In the present novel approach, we tested the hypothesis whether acid-adapted microalgae, initially isolated from non-acidophilic environments, can survive and grow in acid-mine-drainage (AMD) samples. Two acid-adapted microalgal strains, Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, were tested individually or in combination (co-culture) for phenotypic changes during their growth in samples collected from AMD. The acid-adapted microalgae in AMD exhibited a two-fold increase in growth when compared with those grown at pH 3.5 in BBM up to 48 h and then declined. Furthermore, oxidative stress triggered several alterations such as increased cell size, granularity, and enhanced lipid accumulation in AMD-grown microalgae. Especially, the apparent limitation of phosphate in AMD inhibited the uptake of copper and iron in the cultures. Interestingly, growth of the acid-adapted microalgae in AMD downregulated amino acid metabolic pathways as a survival mechanism. This study demonstrates for the first time that acid-adapted microalgae can survive under extreme environmental conditions as exist in AMD by effecting significant phenotypic changes.
- Subject
- AMD remediation; acid-adapted microalgae; phenotypic changes; oxidative stress; metabolomic changes in AMD
- Identifier
- http://hdl.handle.net/1959.13/1447600
- Identifier
- uon:43200
- Identifier
- ISSN:1574-6941
- Rights
- This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS Microbiology Ecology following peer review. The version of record Abinandan, Sudharsanam; Perera, Isiri Adhiwarie; Subashchandrabose, Suresh R.; Venkateswarlu, Kadiyala; Cole, Nicole; Megharaj, Mallavarapu. "Acid-adapted microalgae exhibit phenotypic changes for their survival in acid mine drainage samples”. Published in FEMS Microbiology Ecology Vol. 96, Issue 11 (2020), is available online at: http://dx.doi.org/10.1093/femsec/fiaa113.
- Language
- eng
- Full Text
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