Alpha-diversity is strongly influenced by the composition of other samples when using multiplexed sequencing approaches

Title: Alpha-diversity is strongly influenced by the composition of other samples when using multiplexed sequencing approaches
Creator: Bissett, Andrew; Brown, Mark V.
Relation: Soil Biology and Biochemistry Vol. 127, p. 79-81
Publisher Link: http://dx.doi.org/10.1016/j.soilbio.2018.09.017
Publisher: Elsevier
Resource Type: journal article
Date: 2018
Description: Microbial alpha-diversity, a fundamental ecological concept, is often determined using multiplexed amplicon sequencing approaches. Here we show, using standardized controls and 1879 soil samples, across 21 illumina MiSEQ high throughput sequencing (HTS) runs, that sample alpha-diversity estimates are highly dependent upon the overall diversity of all samples within the sequencing run, a likely result of within sequencing run cross-talk. Cross-talk, therefore, has grave implications for alpha-diversity interpretation in mulitplexed sequencing studies.
Subject: alpha-diversity; MiSEQ; sequencing artefacts; multiplex sequencing; amplicon
Identifier: http://hdl.handle.net/1959.13/1447287
Identifier: uon:43107
Identifier: ISSN:0038-0717
Language: eng
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