- Title
- Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD
- Creator
- Newing, Timothy P.; Oakley, Aaron J.; Miller, Michael; Dawson, Catherine J.; Brown, Simon H. J.; Bouwer, James C.; Tolun, Gokhan; Lewis, Peter J.
- Relation
- Nature Communications Vol. 11, Issue 1, no. 6420
- Publisher Link
- http://dx.doi.org/10.1038/s41467-020-20157-5
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2020
- Description
- In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled complexes, but it was not known how it performed this function. Here, using single particle cryo-electron microscopy, we have determined the structures of Bacillus subtilis RNA polymerase (RNAP) elongation and HelD complexes, enabling analysis of the conformational changes that occur in RNAP driven by HelD interaction. HelD has a 2-armed structure which penetrates deep into the primary and secondary channels of RNA polymerase. One arm removes nucleic acids from the active site, and the other induces a large conformational change in the primary channel leading to removal and recycling of the stalled polymerase, representing a novel mechanism for recycling transcription complexes in bacteria.
- Subject
- bacteria; molecular; DNA replication; cryo-electron microscopy; SDG 12; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1442763
- Identifier
- uon:41790
- Identifier
- ISSN:2041-1723
- Rights
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Language
- eng
- Full Text
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