Isolation, characterisation and detection of breath-derived extracellular vesicles

Title: Isolation, characterisation and detection of breath-derived extracellular vesicles
Creator: Dobhal, Garima; Datta, Amrita; Ayupova, Deanna; Teesdale-Spittle, Paul; Goreham, Renee V.
Relation: Scientific Reports Vol. 10, no. 17381
Publisher Link: http://dx.doi.org/10.1038/s41598-020-73243-5
Publisher: Springer
Resource Type: journal article
Date: 2020
Description: The physical characterisation, capture and detection of extracellular vesicles (EVs) and exosomes derived from breath condensate is reported. Breath-derived EVs were isolated from breath condensate and captured on a gold substrate using two complimentary methods. The characterised and isolated EVs were detected using surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS). EIS was done using aptamers as a targeting moiety and showed a larger change in resistance between dilute concentrations of EVs (less than 7 μg/mL).This is the first report of EVs and exosomes isolated and characterised from breath. In addition, EVs from a non-invasive and easily available source such as breath opens up further avenues in the detection of pulmonary diseases.
Subject: breath analysis; exosome; impedance spectroscopy; metabolism
Identifier: http://hdl.handle.net/1959.13/1438780
Identifier: uon:40715
Identifier: ISSN:2045-2322
Language: eng
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