- Title
- Dynamin regulates L cell secretion in human gut
- Creator
- Sun, Emily WL.; Matusica, Dusan; Wattchow, David A.; McCluskey, Adam; Robinson, Philip J.; Keating, Damien J.
- Relation
- Molecular and Cellular Endocrinology Vol. 535, Issue 15 September 2021, no. 111398
- Publisher Link
- http://dx.doi.org/10.1016/j.mce.2021.111398
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2021
- Description
- Background: The mechanochemical enzyme dynamin mediates endocytosis and regulates neuroendocrine cell exocytosis. Enteroendocrine L cells co-secrete the anorectic gut hormones glucagon-like peptide 1 (GLP-1) and peptide YY (PYY) postprandially and is a potential therapeutic target for metabolic diseases. In the present study, we aimed to determine if dynamin is implicated in human L cell secretion. Methods: Western blot was performed on the murine L cell line GLUTag. Static incubation of human colonic mucosae with activators and inhibitors of dynamin was carried out. GLP-1 and PYY contents of the secretion supernatants were assayed using ELISA. Results and conclusion: s: Both dynamin I and II are expressed in GLUTag cells. The dynamin activator Ryngo 1–23 evoked significant GLP-1 and PYY release from human colonic mucosae while the dynamin inhibitor Dynole 3–42 significantly inhibited release triggered by known L cell secretagogues. Thus, the cell signaling regulator dynamin is able to bi-directionally regulate L cell hormone secretion in the human gut and may represent a novel target for gastrointestinal-targeted metabolic drug development.
- Subject
- dynamin; enteroendocrine cells; GLP-1; PYY; gut hormones
- Identifier
- http://hdl.handle.net/1959.13/1437257
- Identifier
- uon:40290
- Identifier
- ISSN:0303-7207
- Language
- eng
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