- Title
- The effect of arsenic on soil intracellular and potential extracellular β-glucosidase differentiated by chloroform fumigation
- Creator
- Li, Huayong; Tian, Haixia; Liu, Chaoyang; Lu, Guannan; Wang, Ziquan; Tan, Xiangping; Jia, Hanzhong; Megharaj, Mallavarapu; He, Wenxiang
- Relation
- Science of the Total Environment Vol. 727, Issue 20 July 2020, no. 138659
- Publisher Link
- http://dx.doi.org/10.1016/j.scitotenv.2020.138659
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2020
- Description
- Arsenic (As) contamination of soil is a global issue of serious ecological and human health concern. For better use of soil enzymes as biological indicators of As pollution, the response of soil β-glucosidase in different pools of soil (total, intracellular and potential extracellular) to As(V) stress was investigated. Chloroform fumigation method was employed to distinguish the intracellular and potential extracellular β-glucosidase in three soils. The intracellular and potential extracellular β-glucosidase accounted about 79% and 21% of the total β-glucosidase activity in the tested soils. Moreover, it was found that the response of these three enzyme pools to As(V) pollution was different. Under the stress of 400 mg kg−1 As(V), the β-glucosidase activities decreased by 69%, 79%, and 28% for the total, intracellular and potential extracellular pools, respectively. The calculated median ecological dose (ED50) showed the highest value for potential extracellular β-glucosidase (19.55–27.63 mg kg−1 for total, 18.49–27.42 mg kg−1 for intracellular, and 32.27–52.69 mg kg−1 for potential extracellular β-glucosidase). As(V) exhibited an uncompetitive inhibition for total and intracellular β-glucosidase and non-competitive inhibition for potential extracellular enzyme. The inhibition constant (Kiu) is biggest for potential extracellular β-glucosidase among the three enzyme pools (0.61–0.79 mmol L−1 for total, 0.34–0.36 mmol L−1 for intracellular, and 4.01–23.90 mmol L−1 for potential extracellular β-glucosidase). Thus, compared to potential extracellular β-glucosidase, the total and intracellular β-glucosidases are more suitable for their use as sensitive indicators of As(V) pollution.
- Subject
- arsenic; chloroform fumigation; intracellular β-glucosidase; enzyme kinetics; ecological dose; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1434871
- Identifier
- uon:39536
- Identifier
- ISSN:0048-9697
- Language
- eng
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