- Title
- ScNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells
- Creator
- Clark, Stephen J.; Argelaguet, Ricard; Stegle, Oliver; Reik, Wolf; Kapourani, Chantriolnt-Andreas; Stubbs, Thomas M.; Lee, Heather J.; Alda-Catalinas, Celia; Krueger, Felix; Sanguinetti, Guido; Kelsey, Gavin; Marioni, John C.
- Relation
- Nature Communications Vol. 9, Issue 1, p. 781-790
- Publisher Link
- http://dx.doi.org/10.1038/s41467-018-03149-4
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2018
- Description
- Parallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation.
- Subject
- single-cell nucleosome; molecular genetics; transcriptome profiling; sequencing
- Identifier
- http://hdl.handle.net/1959.13/1401140
- Identifier
- uon:34876
- Identifier
- ISSN:2041-1723
- Rights
- © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
- Language
- eng
- Full Text
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