- Title
- Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development
- Creator
- Upton, Rose; Clulow, Simon; Mahony, Michael J.; Clulow, John
- Relation
- Conservation Physiology Vol. 6, no. coy043
- Publisher Link
- http://dx.doi.org/10.1093/conphys/coy043
- Publisher
- Oxford University Press
- Resource Type
- journal article
- Date
- 2018
- Description
- Amphibians are the most threatened vertebrate class globally based on recent rates of decline and extinction. Sperm cryopreservation and other assisted reproductive technologies have the potential to help manage small and threatened populations and prevent extinctions. There are a growing number of reports of recovery of amphibian sperm after cryopreservation, but relatively few published reports of amphibian embryos generated from frozen sperm developing beyond metamorphosis to the adult stage and achieving sexual maturation. In this study on the Eastern dwarf tree frog (Litoria fallax), a temperate amphibian species from eastern Australia, a small number of viable metamorphs and one sexually mature male frog (itself producing sperm) were produced from cryopreserved sperm, demonstrating the capacity of embryos generated from cryopreserved sperm to complete the life cycle to sexual maturity. Low progression rates between developmental stages were not deemed to be due to effects of cryopreservation, since control embryos from unfrozen sperm had a similarly low progression rate through development.
- Subject
- ART; assisted reproductive technologies; conservation; cryopreservation; IVF; sperm
- Identifier
- http://hdl.handle.net/1959.13/1401073
- Identifier
- uon:34867
- Identifier
- ISSN:2051-1434
- Rights
- © The Author(s) 2018. Published by Oxford University Press and the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/4.0), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Language
- eng
- Full Text
- Reviewed
- Hits: 2007
- Visitors: 2273
- Downloads: 308
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 401 KB | Adobe Acrobat PDF | View Details Download |