- Title
- The role of the CopA copper efflux system in acinetobacter baumannii virulence
- Creator
- Alquethamy, Saleh F.; Khorvash, Marjan; Pederick, Victoria G.; Whittall, Jonathan J.; Paton, James C.; Paulsen, Ian T.; Hassan, Karl A.; McDevitt, Christopher A.; Eijkelkamp, Bart A.
- Relation
- International Journal of Molecular Sciences Vol. 20, Issue 3, no. 575
- Publisher Link
- http://dx.doi.org/10.3390/ijms20030575
- Publisher
- MDPI AG
- Resource Type
- journal article
- Date
- 2019
- Description
- Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intrinsic and adapted antibiotic resistance, treatment failure rates are high, which allows this opportunistic pathogen to thrive during infection in immune-compromised patients. A. baumannii can cause infections within a broad range of host niches, with pneumonia and bacteraemia being associated with the greatest levels of morbidity and mortality. Although its resistance to antibiotics is widely studied, our understanding of the mechanisms required for dealing with environmental stresses related to virulence and hospital persistence, such as copper toxicity, is limited. Here, we performed an in silico analysis of the A. baumannii copper resistome, examining its regulation under copper stress. Using comparative analyses of bacterial P-type ATPases, we propose that A. baumannii encodes a member of a novel subgroup of P1B-1 ATPases. Analyses of three putative inner membrane copper efflux systems identified the P1B-1 ATPase CopA as the primary mediator of cytoplasmic copper resistance in A. baumannii. Using a murine model of A. baumannii pneumonia, we reveal that CopA contributes to the virulence of A. baumannii. Collectively, this study advances our understanding of how A. baumannii deals with environmental copper toxicity, and it provides novel insights into how A. baumannii combats adversities encountered as part of the host immune defence.
- Subject
- bacterial; metal ions; P1B-1; P-type ATPases; virulence
- Identifier
- http://hdl.handle.net/1959.13/1400344
- Identifier
- uon:34759
- Identifier
- ISSN:1422-0067
- Rights
- This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
- Language
- eng
- Full Text
- Reviewed
- Hits: 6117
- Visitors: 6567
- Downloads: 156
Collections
Goal 3: Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 2 MB | Adobe Acrobat PDF | View Details Download |