- Title
- Chromatographic behaviour of selected steroids and their inclusion complexes with β-cyclodextrin on octadecylsilica stationary phases with different carbon loads
- Creator
- Zarzycki, Pawel K.; Kulhanek, Kathrin M.; Smith, Roger
- Relation
- Journal of Chromatography A Vol. 955, Issue 1, p. 71-78
- Publisher Link
- http://dx.doi.org/10.1016/S0021-9673(02)00196-6
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2002
- Description
- Retention and separation studies of selected estrogens, progestogens and their inclusion complexes with β-cyclodextrin were conducted using two C₁₈HPLC columns with different carbon loads. The difference in carbon load between investigated octadecylsilica packing materials was about 50%. The mobile phases were composed of a 30% v/v acetonitrile–water mixture without and with addition of β-cyclodextrin at a concentration of 12 mM. The experimental data revealed that retention of the steroids was significantly reduced on the column with the lower carbon load. Moreover, it was found that this column offers better separation power and shorter analysis time at the temperatures studied. However, the calculated values of the retention factor ratios (k0mMCD/k₁₂mMCD) of the steroids were similar for both columns investigated. This observation suggests that the stationary phase structure appears to have little effect on the formation of host–guest complexes if the complexation process is localised to the chromatographic mobile phase. From a practical point of view, when the mobile phase is modified with β-cyclodextrin, the separation of the steroids is strongly influenced by temperature. The best chromatographic conditions were determined for the separation of multicomponent samples on the column with lower carbon load. A possible retention mechanism for components of interest in the presence of macrocyclic additives is discussed.
- Subject
- steroids; cyclodextrins; estrogens; progestogens
- Identifier
- http://hdl.handle.net/1959.13/34012
- Identifier
- uon:3469
- Identifier
- ISSN:0021-9673
- Language
- eng
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