- Title
- Scout-MRM: multiplexed targeted mass spectrometry-based assay without retention time scheduling exemplified by Dickeya dadantii proteomic analysis during plant infection
- Creator
- Rougemont, Blandine; Bontemps Gallo, Sébastien; Ayciriex, Sophie; Carrière, Romain; Hondermarck, Hubert; Lacroix, Jean Marie; Le Blanc, J. C. Yves; Lemoine, Jérôme
- Relation
- Analytical Chemistry Vol. 89, Issue 3, p. 1421-1426
- Publisher Link
- http://dx.doi.org/10.1021/acs.analchem.6b03201
- Publisher
- American Chemical Society
- Resource Type
- journal article
- Date
- 2017
- Description
- Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.
- Subject
- targeted mass spectrometry; plant infection; protein networks; multiple reaction monitoring method; Dickeya dadantii
- Identifier
- http://hdl.handle.net/1959.13/1399193
- Identifier
- uon:34555
- Identifier
- ISSN:0003-2700
- Language
- eng
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