- Title
- Proteomic analysis of human spermatozoa
- Creator
- Nixon, Brett; Dun, Matthew D.; Aitken, R. John
- Relation
- Immune Fertility: Impact of Immune Reactions on Human Fertility p. 3-22
- Publisher Link
- http://dx.doi.org/10.1007/978-3-319-40788-3_1
- Publisher
- Springer
- Resource Type
- book chapter
- Date
- 2017
- Description
- Novel technological innovations in high-resolution mass spectrometry have ushered in a new era in proteomic analyses. Coupled with enhanced methods for cellular and protein pre-fractionation, such developments have enabled the detailed characterization of proteomes from various cell types, including the spermatozoa of a number of species. Collectively, these studies have generated complex inventories consisting of thousands of sperm proteins and served as an important platform for advancing our understanding of sperm biology. In this context, exciting advances have been made into comparative and quantitative approaches that enable sophisticated analysis of the proteomic signature of spermatozoa in different functional states (immature vs. mature, non-capacitated vs. capacitated, fertile vs. infertile). These techniques have helped to define which specific elements of the proteome are of functional significance and improved our understanding of the cascade of post-translational modifications (e.g. phosphorylation, glycosylation, acetylation, proteolytic cleavage) involved in generating a fertilization competent spermatozoon. Such fundamental information holds considerable promise for identifying key biomarkers of male fertility in addition to elements of sperm maturation that might be targeted for fertility regulation both in the context of contraceptive development and therapeutic intervention. In this review, we have sought to present an overview of the use of contemporary proteomics to address many of the long-standing challenges in the field of human sperm biology as well as to speculate on the future clinical applications of these technologies.
- Description
- 2nd ed.
- Subject
- protein spot; male infertility; human sperm; sperm function; sperm protein
- Identifier
- http://hdl.handle.net/1959.13/1392995
- Identifier
- uon:33479
- Identifier
- ISBN:9783319407869
- Language
- eng
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