- Title
- Microwave irradiation enhances the in vitro antifungal activity of citrus by-product aqueous extracts against Alternaria alternata
- Creator
- Papoutsis, Konstantinos; Vuong, Quan V.; Tesoriero, Len; Pristijono, Penta; Stathopoulos, Costas E.; Gkountina, Stela; Lidbetter, Fiona; Bowyer, Michael C.; Scarlett, Christopher J.; Golding, John B.
- Relation
- International Journal of Food Science and Technology Vol. 53, Issue 6, p. 1510-1517
- Publisher Link
- http://dx.doi.org/10.1111/ijfs.13732
- Publisher
- Wiley-Blackwell
- Resource Type
- journal article
- Date
- 2018
- Description
- The effect of two lemon by-product aqueous extracts at different concentrations (14, 7, 3.5 and 1 mg mL-1) was tested against the in vitro growth of Alternaria alternata. Prior to extraction, one batch of by-product was dehydrated by freeze-drying (untreated by-product), while the other batch was treated by microwave irradiation in conjunction with freeze-drying (microwave-treated by-product). High-performance liquid chromatography (HPLC) was employed for the identification of individual phenolic compounds with potent antifungal activities. Both lemon by-product aqueous extracts inhibited the mycelial growth and suppressed the spore germination of the fungus in a concentration-dependent manner. In general, the extracts obtained from the microwave-treated lemon by-product displayed enhanced antifungal activity than those obtained from the untreated one. Scanning electron microscopy (SEM) revealed that both lemon by-product extracts affected the hyphal morphology of the fungus. The antifungal activity of the extracts was attributed to their phenolic acid and ascorbic acid contents.
- Subject
- antifungal activity; conidia; lemon waste; mycelial growth inhibition; phenolic compounds; sweet cherry
- Identifier
- http://hdl.handle.net/1959.13/1392556
- Identifier
- uon:33418
- Identifier
- ISSN:0950-5423
- Language
- eng
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