- Title
- Conditionally reprogrammed primary airway epithelial cells maintain morphology, lineage and disease specific functional characteristics
- Creator
- Martinovich, Kelly M.; Iosifidis, Thomas; Buckley, Alysia G.; Looi, Kevin; Ling, Kak-Ming; Sutanto, Erika N.; Kicic-Starcevich, Elizabeth; Garratt, Luke W.; Shaw, Nicole C.; Montgomery, Samuel; Lannigan, Fracis J.; Knight, Darryl A.; Kicic, Anthony; Stick, Stephen M.
- Relation
- Scientific Reports Vol. 7, Issue 1, no. 17971
- Publisher Link
- http://dx.doi.org/10.1038/s41598-017-17952-4
- Publisher
- Nature Publishing
- Resource Type
- journal article
- Date
- 2017
- Description
- Current limitations to primary cell expansion led us to test whether airway epithelial cells derived from healthy children and those with asthma and cystic fibrosis (CF), co-cultured with an irradiated fibroblast feeder cell in F-medium containing 10 µM ROCK inhibitor could maintain their lineage during expansion and whether this is influenced by underlying disease status. Here, we show that conditionally reprogrammed airway epithelial cells (CRAECs) can be established from both healthy and diseased phenotypes. CRAECs can be expanded, cryopreserved and maintain phenotypes over at least 5 passages. Population doublings of CRAEC cultures were significantly greater than standard cultures, but maintained their lineage characteristics. CRAECs from all phenotypes were also capable of fully differentiating at air-liquid interface (ALI) and maintained disease specific characteristics including; defective CFTR channel function cultures and the inability to repair wounds. Our findings indicate that CRAECs derived from children maintain lineage, phenotypic and importantly disease-specific functional characteristics over a specified passage range.
- Subject
- airway epithelial cells; primary cells; asthma; cystic fibrosis; disease-specific functional characteristics
- Identifier
- http://hdl.handle.net/1959.13/1384572
- Identifier
- uon:32103
- Identifier
- ISSN:2045-2322
- Rights
- © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Language
- eng
- Full Text
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