- Title
- Functional identification of a novel transcript variant of INPP4B in human colon and breast cancer cells
- Creator
- Croft, Amanda; Guo, Su Tang; Sherwin, Simonne; Farrelly, Margaret; Yan, Xu Guang; Zhang, Xu Dong; Jiang, Chen Chen
- Relation
- Biochemical and Biophysical Research Communications Vol. 485, Issue 1, p. 47-53
- Publisher Link
- http://dx.doi.org/10.1016/j.bbrc.2017.02.012
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2017
- Description
- The 4-phosphatase Inositol polyphosphate 4-phosphatase II (INPP4B) is a regulator of the PI3K signalling pathway and functions to suppress or promote activation of downstream kinases depending on cell type and context. Here we report the identification of a novel small transcript variant of INPP4B (INPP4B-S) that has a role in promoting proliferation of colon and breast cancer cells. INPP4B-S differed from full length INPP4B (INPP4B-FL) by the insertion of a small exon between exons 15 and 16 and the deletion of exons 20-24. Nevertheless, INPP4B-S retained all the functional domains of INPP4B-FL and was similarly located to the cytoplasm. Overexpression of INPP4B-S increased, whereas selective knockdown of INPP4B-S reduced the rate of proliferation in HCT116 and MCF-7-cells. These results warrant further investigation of the role INPP4B-S in activation of downstream kinases and in regulation of cancer pathogenesis.
- Subject
- INPP4B; transcript variant; colon cancer; breast cancer
- Identifier
- http://hdl.handle.net/1959.13/1355681
- Identifier
- uon:31508
- Identifier
- ISSN:0006-291X
- Language
- eng
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