Development of a whole cell biosensor for the detection of inorganic mercury

Title: Development of a whole cell biosensor for the detection of inorganic mercury
Creator: Mahbub, Khandaker Rayhan; Krishnan, Kannan; Naidu, Ravi; Megharaj, Mallavarapu
Relation: Environmental Technology and Innovation Vol. 8, Issue November 2017, p. 64-70
Publisher Link: http://dx.doi.org/10.1016/j.eti.2017.04.003
Publisher: Elsevier
Resource Type: journal article
Date: 2017
Description: A whole cell bacterial biosensor was developed by modification of chromosomal DNA of an environmental bacterial isolate Sphingobium SA2. The sensing element contained green fluorescence protein gene gfp fused to short segment of merA gene of Sphingobium SA2. The sensing element was introduced into electro-competent cells of Sphingobium SA2, where it integrated into the bacterial DNA due to homologous recombination. The transformed cells were able to produce green fluorescence in 5 h in the presence of nano-molar concentrations of mercury. A linear positive correlation was observed between 0–40 nm Hg and fluorescence intensity.
Subject: bioavailable metal; gfp; detection; toxicity; Sphingobium
Identifier: http://hdl.handle.net/1959.13/1355425
Identifier: uon:31459
Identifier: ISSN:2352-1864
Language: eng
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