- Title
- Expression of ciliated bronchial epithelium 1 during human spermatogenesis
- Creator
- Pleuger, Christiane; Fietz, Daniela; Hartmann, Katja; Schuppe, Hans-Christian; Weidner, Wolfgang; Kliesch, Sabine; Baker, Mark; O'Bryan, Moira K.; Bergmann, Martin
- Relation
- Funding BodyNHMRCGrant Number1058356 http://purl.org/au-research/grants/nhmrc/1058356
- Relation
- Fertility and Sterility Vol. 108, Issue 1, p. 47-54
- Publisher Link
- http://dx.doi.org/10.1016/j.fertnstert.2017.05.019
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2017
- Description
- Objective: To define the precise cellular localization of ciliated bronchial epithelium 1 (CBE1) in the human testis and test its relationship to impaired spermatogenesis. Design: Gene expression analysis, and histologic and immunohistochemical evaluation. Setting: University research laboratories and andrologic outpatient clinic. Patient(s): Forty-three human testicular biopsies: 12 biopsies showing normal spermatogenesis (NSP), 8 with maturation arrest at level of spermatocytes (STA), 8 with maturation arrest at level of spermatids (SDA), 4 with scattered elongating spermatids, and 12 with Sertoli cell-only syndrome, with an additional 5 semen samples from healthy donors. Intervention(s): None. Main Outcome Measure(s): Evaluation of CBE1 expression in normal as well as impaired spermatogenesis on mRNA (quantitative reverse-transcription polymerase chain reaction and in situ hybridization) and protein level (immunohistochemistry, Western blot analysis). Result(s): In normal spermatogenesis, CBE1 mRNA was expressed in late pachytene spermatocytes, and the protein was localized within the flagellum of elongating spermatids from stage V up to the spermiation in stage II. Immunoelectron microscopy showed CBE1 clearly associated with microtubules at the manchette, the head-tail coupling apparatus, and the flagellum, but the protein was absent in spermatozoa. Compared with normal spermatogenesis, CBE1 mRNA was statistically significantly reduced in samples with a maturation arrest at the level of round spermatids and primary spermatocytes, and was absent in samples showing Sertoli cell-only syndrome. CBE1 protein was completely missing in SDA samples showing few elongating spermatids. Conclusion(s): Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. The absence of CBE1 in spermatozoa suggests that CBE1 is important for the spermatid development but not for the maintenance of mature spermatozoa as a component of the flagellum.
- Subject
- intraflagellar transport; intramanchette transport; male fertility; microtubules; spermiogenesis
- Identifier
- http://hdl.handle.net/1959.13/1355160
- Identifier
- uon:31426
- Identifier
- ISSN:0015-0282
- Language
- eng
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