- Title
- Organ culture and whole mount immunofluorescence staining of mouse Wolffian ducts
- Creator
- Kumar, Manish; Tanwar, Pradeep
- Relation
- NHMRC & ARC
- Relation
- Journal of Visualized Experiments (JoVE) , Issue 119
- Publisher Link
- http://dx.doi.org/10.3791/55134
- Publisher
- Journal of Visualized Experiments
- Resource Type
- journal article
- Date
- 2017
- Description
- We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.
- Subject
- developmental Biology; epididymis; organ culture; immunostaining; Wolffian duct; whole mount; in vitro
- Identifier
- http://hdl.handle.net/1959.13/1352511
- Identifier
- uon:30902
- Identifier
- ISSN:1940-087X
- Language
- eng
- Full Text
- Reviewed
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| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 1 MB | Adobe Acrobat PDF | View Details Download |