- Title
- Activation of Xer-recombination at dif: structural basis of the FtsKγ-XerD interaction
- Creator
- Keller, Andrew N.; Xin, Yue; Boer, Stephanie; Reinhardt, Jonathan; Baker, Rachel; Arciszewska, Lidia K.; Lewis, Peter J.; Sherratt, David J.; Löwe, Jan; Grainge, Ian
- Relation
- NHMRC.1005697 and ARC FT120100153 http://purl.org/au-research/grants/arc/FT120100153
- Relation
- Scientific Reports Vol. 6, no. 33357
- Publisher Link
- http://dx.doi.org/10.1038/srep33357
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2016
- Description
- Bacterial chromosomes are most often circular DNA molecules. This can produce a topological problem; a genetic crossover from homologous recombination results in dimerization of the chromosome. A chromosome dimer is lethal unless resolved. A site-specific recombination system catalyses this dimer-resolution reaction at the chromosomal site dif. In Escherichia coli, two tyrosine-family recombinases, XerC and XerD, bind to dif and carry out two pairs of sequential strand exchange reactions. However, what makes the reaction unique among site-specific recombination reactions is that the first step, XerD-mediated strand exchange, relies on interaction with the very C-terminus of the FtsK DNA translocase. FtsK is a powerful molecular motor that functions in cell division, co-ordinating division with clearing chromosomal DNA from the site of septation and also acts to position the dif sites for recombination. This is a model system for unlinking, separating and segregating large DNA molecules. Here we describe the molecular detail of the interaction between XerD and FtsK that leads to activation of recombination as deduced from a co-crystal structure, biochemical and in vivo experiments. FtsKγ interacts with the C-terminal domain of XerD, above a cleft where XerC is thought to bind. We present a model for activation of recombination based on structural data.
- Subject
- DNA; bacterial chromosomes; genetic; structural data
- Identifier
- http://hdl.handle.net/1959.13/1346105
- Identifier
- uon:29783
- Identifier
- ISSN:2045-2322
- Rights
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Language
- eng
- Full Text
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