- Title
- Microbial diversity and hydrocarbon degrading gene capacity of a crude oil field soil as determined by metagenomics analysis
- Creator
- Abbasian, Firouz; Palanisami, Thavamani; Megharaj, Mallavarapu; Naidu, Ravi; Lockington, Robin; Ramadass, Kavitha
- Relation
- Biotechnology Progress Vol. 32, Issue 3, p. 638-648
- Publisher Link
- http://dx.doi.org/10.1002/btpr.2249
- Publisher
- Wiley-Blackwell Publishing
- Resource Type
- journal article
- Date
- 2016
- Description
- Soils contaminated with crude oil are rich sources of enzymes suitable for both degradation of hydrocarbons through bioremediation processes and improvement of crude oil during its refining steps. Due to the long term selection, crude oil fields are unique environments for the identification of microorganisms with the ability to produce these enzymes. In this metagenomic study, based on Hiseq Illumina sequencing of samples obtained from a crude oil field and analysis of data on MG-RAST, Actinomycetales (9.8%) were found to be the dominant microorganisms, followed by Rhizobiales (3.3%). Furthermore, several functional genes were found in this study, mostly belong to Actinobacteria (12.35%), which have a role in the metabolism of aliphatic and aromatic hydrocarbons (2.51%), desulfurization (0.03%), element shortage (5.6%), and resistance to heavy metals (1.1%). This information will be useful for assisting in the application of microorganisms in the removal of hydrocarbon contamination and/or for improving the quality of crude oil.
- Subject
- metagenomic study; crude oil well; microbial diversity; alkanes; aromatic hydrocarbons
- Identifier
- http://hdl.handle.net/1959.13/1344348
- Identifier
- uon:29405
- Identifier
- ISSN:8756-7938
- Rights
- This is the peer reviewed version of the following article: Abbasian, F., Palanisami, T., Megharaj, M., Naidu, R., Lockington, R. and Ramadass, K. (2016), Microbial diversity and hydrocarbon degrading gene capacity of a crude oil field soil as determined by metagenomics analysis. Biotechnology Progress, 32: 638–648, which has been published in final form at http://dx.doi.org/10.1002/btpr.2249. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
- Language
- eng
- Full Text
- Reviewed
- Hits: 6106
- Visitors: 7298
- Downloads: 826
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Author final version | 988 KB | Adobe Acrobat PDF | View Details Download |