- Title
- Vitamin D receptor genotype modulates the correlation between vitamin D and circulating levels of let-7a/b and vitamin D intake in an elderly cohort
- Creator
- Beckett, Emma Louise; Martin, Charlotte; Duesing, Konsta; Jones, Patrice; Furst, John; Yates, Zoe; Veysey, Martin; Lucock, Mark
- Relation
- Journal of Nutrigenetics and Nutrigenomics Vol. 7, p. 264-273
- Publisher Link
- http://dx.doi.org/10.1159/000381676
- Publisher
- S. Karger AG
- Resource Type
- journal article
- Date
- 2014
- Description
- Background and Aims: Circulating microRNAs (miRNAs) are linked to disease and are potential biomarkers. Vitamin D may modulate miRNA profiles, and vitamin D status has been linked to risk of disease, including cardiovascular disease and cancers. We hypothesise that genotypic variance influences these relationships. We examined the correlations between vitamin D intake and circulating levels of the miRNAs let-7a/b, and the involvement of two common vitamin D receptor (VDR) polymorphisms, BsmI and ApaI. Methods: Two hundred participants completed food frequency and supplement questionnaires, and were assayed for circulating let-7b expression by qPCR. Polymorphisms were detected using restriction fragment length polymorphism-PCR. Results: let-7b expression negatively correlated with vitamin D intake (rs = -0.20, p = 0.005). The magnitude and direction of correlation were maintained in the presence of the BsmI restriction site (rs = -0.27, p = 0.0005). However, in the absence of BsmI restriction site, the direction of the correlation was reversed (rs = +0.319, p = 0.0497). These correlations were significantly different (z-score = 2.64, p = 0.0085). The correlation between vitamin D intake and let-7a was only significant in those without the ApaI restriction site. Conclusions: The correlation between vitamin D intake and let-7a/b expression in this cohort varies with VDR genotype. This study highlights the importance of considering underlying genotypic variance in miRNA expression studies and in nutritional epigenetics generally.
- Subject
- let-7 microRNA; vitamin D; vitamin D receptor; <i>Bsm</i>I restriction site; <i>Apa</i>I restriction site; Nutrigenomics
- Identifier
- http://hdl.handle.net/1959.13/1342326
- Identifier
- uon:28948
- Identifier
- ISSN:1661-6499
- Rights
- This is the peer-reviewed but unedited manuscript version of the following article: Beckett, Emma Louise; Martin, Charlotte; Duesing, Konsta; Jones, Patrice; Furst, John; Yates, Zoe; Veysey, Martin; Lucock, Mark “Vitamin D receptor genotype modulates the correlation between vitamin D and circulating levels of let-7a/b and vitamin D intake in an elderly cohort”. Journal of Nutrigenetics and Nutrigenomics Vol. 7, p. 264-273 (2014). The final, published version is available at http://www.karger.com/?doi=10.1159/000381676
- Language
- eng
- Full Text
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