- Title
- Properties of sodium currents in neonatal and young adult mouse superficial dorsal horn neurons
- Creator
- Tadros, Melissa A.; Farrell, Kristen E.; Graham, Brett A.; Brichta, Alan M.; Callister, Robert J.
- Relation
- NHMRC.569206 | NHMRC|631000 http://purl.org/au-research/grants/nhmrc/631000
- Relation
- Molecular Pain Vol. 11, Issue 1
- Publisher Link
- http://dx.doi.org/10.1186/s12990-015-0014-5
- Publisher
- BioMed Central
- Resource Type
- journal article
- Date
- 2015
- Description
- Background: Superficial dorsal horn (SDH) neurons process nociceptive information and their excitability is partly determined by the properties of voltage-gated sodium channels. Recently, we showed the excitability and action potential properties of mouse SDH neurons change markedly during early postnatal development. Here we compare sodium currents generated in neonate (P0-5) and young adult (≥P21) SDH neurons. Results: Whole cell recordings were obtained from lumbar SDH neurons in transverse spinal cord slices (CsF internal, 32°C). Fast activating and inactivating TTX-sensitive inward currents were evoked by depolarization from a holding potential of 100mV. Poorly clamped currents, based on a deflection in the IV relationship at potentials between 60 and 50mV, were not accepted for analysis. Current density and decay time increased significantly between the first and third weeks of postnatal development, whereas time to peak was similar at both ages. This was accompanied by more subtle changes in activation range and steady state inactivation. Recovery from inactivation was slower and TTX-sensitivity was reduced in young adult neurons. Conclusions: Our study suggests sodium channel expression changes markedly during early postnatal development in mouse SDH neurons. The methods employed in this study can now be applied to future investigations of spinal cord sodium channel plasticity in murine pain models.
- Subject
- development; activation; spinal cord; pain; action potential
- Identifier
- http://hdl.handle.net/1959.13/1337999
- Identifier
- uon:27947
- Identifier
- ISSN:1744-8069
- Rights
- © 2015 Tadros et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Language
- eng
- Full Text
- Reviewed
- Hits: 1750
- Visitors: 2271
- Downloads: 251
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 5 MB | Adobe Acrobat PDF | View Details Download |