- Title
- Dynamin 2 is essential for mammalian spermatogenesis
- Creator
- Redgrove, Kate A.; Bernstein, Ilana R.; Pye, Victoria J.; Mihalas, Bettina P.; Sutherland, Jessie M.; Nixon, Brett; McCluskey, Adam; Robinson, Phillip J.; Holt, Janet E.; McLaughlin, Eileen A.
- Relation
- Scientific Reports Vol. 6
- Publisher Link
- http://dx.doi.org/10.1038/srep35084
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2016
- Description
- The dynamin family of proteins play important regulatory roles in membrane remodelling and endocytosis, especially within brain and neuronal tissues. In the context of reproduction, dynamin 1 (DNM1) and dynamin 2 (DNM2) have recently been shown to act as key mediators of sperm acrosome formation and function. However, little is known about the roles that these proteins play in the developing testicular germ cells. In this study, we employed a DNM2 germ cell-specific knockout model to investigate the role of DNM2 in spermatogenesis. We demonstrate that ablation of DNM2 in early spermatogenesis results in germ cell arrest during prophase I of meiosis, subsequent loss of all post-meiotic germ cells and concomitant sterility. These effects become exacerbated with age, and ultimately result in the demise of the spermatogonial stem cells and a Sertoli cell only phenotype. We also demonstrate that DNM2 activity may be temporally regulated by phosphorylation of DNM2 via the kinase CDK1 in spermatogonia, and dephosphorylation by phosphatase PPP3CA during meiotic and post-meiotic spermatogenesis.
- Subject
- dynamin 2; spermatogenesis; formation; function
- Identifier
- http://hdl.handle.net/1959.13/1336959
- Identifier
- uon:27739
- Identifier
- ISSN:2045-2322
- Rights
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Language
- eng
- Full Text
- Reviewed
- Hits: 2052
- Visitors: 2267
- Downloads: 228
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 2 MB | Adobe Acrobat PDF | View Details Download |