- Title
- Boronate probes for the detection of hydrogen peroxide release from human spermatozoa
- Creator
- Purdey, Malcolm S.; Connaughton, Haley S.; Whiting, Sara; Schartner, Erik P.; Monro, Tayna M.; Thompson, Jeremy G.; Aitken, R. John; Abell, Andrew D.
- Relation
- ARC.LP110200736, ARC.DP110103951 & ARC.FL130100044 http://purl.org/au-research/grants/arc/DP110103951
- Relation
- Free Radical Biology and Medicine Vol. 81, Issue April 2015, p. 69-76
- Publisher Link
- http://dx.doi.org/10.1016/j.freeradbiomed.2015.01.015
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2015
- Description
- Human spermatozoa are compromised by production of reactive oxygen species (ROS), and detection of ROS in spermatozoa is important for the diagnosis of male infertility. The probes 2',7'-dichlorohydrofluorescein diacetate (DCFH), dihydroethidium (DHE), and MitoSOX red (MSR) are commonly used for detecting ROS by flow cytometry; however, these probes lack sensitivity to hydrogen peroxide (H2O2), which is particularly damaging to mammalian sperm cells. This study reports the synthesis and use of three aryl boronate probes, peroxyfluor-1 (PF1), carboxyperoxyfluor-1, and a novel probe, 2-(2-ethoxyethoxy)ethoxyperoxyfluor-1 (EEPF1), in human spermatozoa. PF1 and EEPF1 were effective at detecting H₂O₂ and peroxynitrite (ONOO-) produced by spermatozoa when stimulated with menadione or 4-hydroxynonenal. EEPF1 was more effective at detection of ROS in spermatozoa than DCFH, DHE, or MSR; furthermore it distinguished poorly motile sperm as shown by greater ROS production. EEPF1 should therefore have a significant role in the diagnosis of oxidative stress in male infertility, cryopreservation, age, lifestyle, and exposure to environmental toxicants.
- Subject
- hydrogen peroxide; reactive oxygen species; human spermatozoa; organic synthesis; aryl boronate; free radicals
- Identifier
- http://hdl.handle.net/1959.13/1335138
- Identifier
- uon:27394
- Identifier
- ISSN:0891-5849
- Language
- eng
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